|
KMID : 0390220030140010113
|
|
Journal of Clinical Otolaryngology, Head and Neck Surgery
2003 Volume.14 No. 1 p.113 ~ p.117
|
|
|
The Role of Insulin-Like Growth Factor I and Binding Protein in Cholesteatoma Fibroblasts
|
|
Lee Chang-Hoon
Cho Chin-Saeng
Park Kyung-You
Kim Joon-Woo
Lee Byung-Kwon
Lee Jae-Soo
|
|
|
Abstract
|
|
|
|
Background and Objectives：Insulin like growth factor-I is an important growth regulator for epidermis and its activity is regulated by specific insulin like growth factor binding protein. Authors observed the expression of mRNA of Insulin-like Growth FactorI (IGF-I) and mRNA of Insulin-like Growth Factor Binding Protein (IGFBP) in fibroblast of patient with cholesteatoma to find out the function of fibroblast in the progress of cholesteatoma. Materials and Method：During the operation in middle ears, the cholesteatoma and normal skin tissue were extracted from 10 patients with Cholesteatoma. Some cytokines were added such as Interferonβ, Interferonγ and TNFα to those extraced fibroblast. We analyzed the expression of IGF-I, IGFBP-3, and IGFBP-5 by semi-quantative RT-PCR. Results：After stimulation by inflammatory cytokine, the expression of IGF-I from cholesteatoma was significantly increased ,while the expression of IGFBP-5 was significantly reduced. Conclusion：These results indicate that cholesteatoma fibroblast could play an important role in the proliferation of cholesteatoma tissue.
|
|
|
KEYWORD
|
|
|
Cholesteatoma, Fibroblasts, Cytokines
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|